عنوان مقاله [English]
نویسندگان [English]چکیده [English]
In recent years efforts to find bioactive compounds from live organisms especially marine animals have been increased. In the present study, the anticancer and cytotoxic effects of sea cucumber body walls (Holothuria leucospilota) were investigated. For this purpose, sea cucumbers were collected from Larak Island at depths of 10 to 30 m and extraction process was done with methanol and diethyl ether solvent which then concentrated by rotary evaporator (40℃) following lyophilization with vacuum freeze dryer. XTT method was used to investigate anticancer and cytotoxic effects of body wall extracts. The results showed that the methanolic extract could prevent proliferation of human oral epidermoid carcinoma cells (KB) at concentrations of 100 and 500 μg/ml. The diethyl etheric extract also could prevent proliferation of KB at 500 μg/ml concentration. Overall result showed that sea cucumber body wall had a strong cytotoxic effect on normal cell line (Human embryonic kidney cell [HEK]) which can be used as potent cytotoxic material. However these extracts did not show significant therapeutic value against KB cells.
Althunibat O.Y., Hashim R.B., Taher M., Daud J.M., Ikeda M.A. and Zali B.I., 2009. In vitro antioxidant and anti proliferative activities of three Malaysian sea cucumber species.European Journal of Scientific Research, 37 (3): 376-387.
Atkinson J.C., Harvey K.E. and Domingo D.L., 2008. Oral and dental phenotype of dyskeratosis congenita. Oral Disease, 14: 419–427.
Bordbar S., Anwar F. and Saari N., 2011. High-value components and bioactives from sea cucumbers for functional foods. Marine Drugs, 9: 1761-1805.
Chludil, H.D. Murray, A.P. Seldes, A.M. and Maier, M.S., 2003.Biologically active triterpene glycosides from sea cucumbers (holothuroidea, echinodermata). Studies in natural products chemistry, 28 (Part I): 587-616.
Farouk A.A., Ghouse F.A.H. and Ridzwan B.H., 2007. New bacterial species isolated from Malaysian sea cucumbers with optimized secreted antibacterial activity. Americam Journal of Biochemistry and Biotehcnology, 3: 60-65.
Faulker J., 1996. Marine natural products. Natural Products, 13: 75- 125.
Hamel J.F. and Mercer A., 1996. Evidence of chemical communication during the Gametogenesis of Holothuroids. Ecology, 77 (5): 1600-1616.
Hua H., ling L., Yangyang-Hua Y., Xiao-Hua W. and Min-Xiang P., 2012. Tiriterpen glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Chinese Herbal Medicines, 4(3): 183-188.
Kraljevic S., Sedic M., Scott M., Gehrig P., Schlapbach R. and Pavelic K., 2006. Casting light on molecular events underlying anti-cancer drug treatment: what can be seen from the proteomics point of view. Cancer Treatment Reviews, 32(8): 619-29.
Naik C.G., Kamat S.Y., Parameswaran P.S., Das B., Bhattacharya S., Ramani P., Bhakuni D.S., Goel A.K., Jain S. and Srimal R.C., 1989. Bioactivity of marine organisms. IV- Screening of some marine animals from the Indian Ocean.