مطالعه اثرات ضد باکتریایی برخی از عصاره‌های گیاهی بر باکتری یرسینیا راکری در شرایط آزمایشگاهی Yersinia ruckeri

نویسندگان

1 موسسه تحقیقات علوم شیلاتی کشور، تهران

2 پژوهشکده اکولوژی دریای خزر، ساری، ایران

3 دانشگاه شهرکرد

چکیده

به دنبال افزایش روز افزون مقاومت­های باکتریایی نسبت به آنتی بیوتیک­های تجاری رایج، گرایش به استفاده از گیاهانی با خواص ضدمیکروبی افزایش یافته است. در این مطالعه اثر ضد باکتریایی عصاره گیاهان گلپر (Heracleum persicum)، رازیانه (Foeniculum Vulgare)، اسفرزه (Plantago psyllium)، چای سبز (Camellia Sinensis)، گزنه (Urtica dioica) و سیر (Allium sativum) بر باکتری یرسینیا راکری (Yersinia ruckeri) در شرایط آزمایشگاهی مورد بررسی قرار گرفت. برای تعیین قدرت ضد باکتریایی عصاره­ها از روش­های استاندارد، انتشار در دیسک، تعیین حداقل غلظت مهارکنندگی رشد (MIC) و حداقل غلظت باکتری کشی (MBC) استفاده گردید و فورازولیدون به عنوان نمونه استاندارد به کار گرفته شد. حداقل غلظت مهارکنندگی رشد عصاره­های گلپر، رازیانه، اسفرزه، چای سبز، گزنه و سیر برای باکتری یرسینیا راکری به ترتیب 400، 75، 250، 250، 75 و 150 میکروگرم بر میلی­لیتر، مقادیر حداقل غلظت کشندگی عصاره­های مذکور به ترتیب 610، 100،500، 250، 150، 250 میکروگرم بر میلی­لیتر و قطر هاله مهار رشد باکتری به ترتیب 6/0±6/17 ،2/1±6/23، 9/0± 4/20، 7/0±8/18، 3/1±2/21 و 1/1±6/22 میلی­متر بدست آمد. در این مطالعه عصاره­های رازیانه، گزنه و سیر اثرات ضد باکتریایی مناسبی را روی باکتری یرسینیا راکری از خود نشان دادند. بنابراین، عصاره های گیاهی رازیانه، گزنه و سیر پس از انجام مطالعات تکمیلی، می­تواند جایگزین مناسبی برای آنتی بیوتیک­های تجاری رایج به منظور درمان عفونت­های سیستماتیک ناشی از باکتری یرسینیا راکری باشند. 

کلیدواژه‌ها


عنوان مقاله [English]

Study the antibacterial effect some of the herbal extracts on Yersinia ruckeri in invitro

چکیده [English]

Due to increased bacterial resistance to common antibiotics, tendency towards using herbal extracts is increasing. In this study, antibacterial effects of the extracts of Heracleum persicum, Foeniculum vulgare, Plantago psyllium, Camellia Sinensis, Urtica dioica and Allium sativum on Yersinia ruckeri were investigated in vitro condition. The disc diffusion method, minimum bacteriocidal concentration (MBC) and determination of minimum inhibitory concentration (MIC) of the extracts against Y. ruckeri were employed to determination of antibacterial effects of extracts and furasolidon were used as standard. The MIC of extract of Heracleum persicum, F. vulgare, P. psyllium, C. Sinensis, U. dioica and A. sativum were 400, 75, 250, 250, 75 and 150 (μg/ml), respectively, MBC of these extracts was 610, 100, 500, 250, 150 and 250 (μg/ml) respectively, and diameter of zone of inhibition were 17.6±0.6 (mm), 23.6± 1.2, 20.4 ± 0.9, 18.8 ± 0.7, 21.2 ± 1.3 and 22.6 ± 1.1 (mm) were, respectively, against Y. ruckeri. Results of present study demonstrated that the extracts of F. vulgare, U. dioica and A. sativum were appropriate antibacterial agents against Y. ruckeri. Based on the result of this study, using these herbal extracts were suitable alternative than common antibiotics to treatment systematic infections caused by Y. ruckeri. 

کلیدواژه‌ها [English]

  • Antibacterial effects
  • Herbal essential extracts
  • Yersinia ruckeri
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