تاثیر مکمل معدنی نانوذره اکسید منگنز بر عملکرد رشد و یاخته‎های خونی بچه‏ ماهی انگشت‏ قد قزل‏ آلای رنگین کمان (Oncorhynchus mykiss Walbaum 1792)

نویسندگان

1 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، صندوق پستی: 775-14515، تهران، ایران.

چکیده

این پژوهش به منظور بررسی کاربرد نانوذره اکسید منگنز (Mn2O3) به عنوان مکمل معدنی غذایی بر شاخص‏های رشد و گلبول‌های قرمز و سفید بچه ماهی قزل‏آلای رنگین‏کمان (Oncorhynchus mykiss) طراحی گردید. تعداد 450 قطعه بچه ماهی قزل‎آلا با میانگین وزن اولیه 3/0±1/9 گرم پس از سازگاری با شرایط استخر به صورت تصادفی در قالب 5 تیمار هر یک با سه تکرار درون استخرهای جریان باز تقسیم شدند. غذای پایه به عنوان تیمار شاهد بدون افزودن مکمل معدنی منگنز آماده گردید. سایر غذاهای آزمایشی با افزودن 4، 8 و 12 میلی‏گرم بر کیلوگرم نانوذرات اکسید منگنز و 12 میلی‏گرم بر کیلوگرم سولفات منگنز (به عنوان شاهد مثبت) آماده شدند. ماهیان به مدت 8 هفته در شرایط آزمایشی با جیره‏های غذایی اختصاصی تغذیه شده و در انتها فاکتورهای رشد همراه با ویژگی‏های اریتروسیتی و لکوسیتی با خونگیری از طریق قطع ساقه دمی تعیین گردید. نتایج نشان داد که اختلاف معنی‏داری در میزان افزایش وزن، ضریب رشد ویژه، نرخ کارایی پروتئین و همچنین ضریب چاقی ماهیان بین تیمارهای مختلف پژوهش حاضر وجود ندارد. این در حالی است که تعداد گلبول‎های قرمز، میزان هموگلوبین و سطح هماتوکریت ماهیان پس از تغذیه با 12 میلی‏گرم بر کیلوگرم نانوذره اکسید منگنز در جیره به شکل معنی‏داری بیشتر از ماهیان سایر تیمارهای آزمایشی بود (p<0.05)؛ ولی تفاوت معنی‏داری در بررسی اندیس‏های گلبول قرمز شامل حجم متوسط گلبولی، مقدار متوسط هموگلوبین گلبولی و غلظت هموگلوبین داخل گلبولی بین تیمارها به دست نیامد. همچنین اختلاف معنی‏داری در متغیرهای فوق بین ماهیان تغذیه شده با غذای حاوی سولفات منگنز و ماهیان در تیمار شاهد ثبت نشد. یافته‏های پژوهش حاضر نشان داد که استفاده از نانوذره اکسید منگنز منجر به افرایش شاخص‎های رشد و شاخص‏های خونی بچه‏ماهی قزل‏آلای رنگین‏کمان شامل تعداد گلبول‏های قرمز، میزان هموگلوبین و درصد هماتوکریت گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of manganese oxide nanoparticle on growth performance and blood cells of rainbow trout (Oncorhynchus mykiss Walbaum 1792) fingerlings

نویسندگان [English]

  • Z Mohammadi 1
  • H Rajabi 2
چکیده [English]

This study was aimed to use nano manganese oxide (Mn2O3) as a mineral premix on growth parameters followed by the erythrocyte and leukocyte properties of rainbow trout (Oncorhynchus mykiss) fingerlings. 450 specimens of rainbow trout fingerlings with an average weight of 9.1±0.3 g after acclimation to the experimental condition were randomly distributed in triplicate to five treatments of raceways ponds with open flow. Basal diet as control treatment was prepared by adding no supplemental manganese mineral. Other experimental diets were prepared by adding 4, 8, 12 mg and 12 mg nano manganese oxide followed by 12 mg manganese sulfate (as positive control treatment) to each kg diet. The Fish were fed for 8 weeks under experimental conditions with the corresponding diets. At the end of feeding trial, growth performance followed by the hematological parameters were determined by cutting the caudal peduncle (n=5). Results demonstrated no significant differences in final weigh, specific growth rate, protein efficiency ratio, and condition factor of the fish among the treatments. However, erythrocytes, hemoglobin, and hematocrit of fish fed by 12 mg kg-1 nanoparticle manganese oxide were significantly higher than fish in other treatments (p<0.05), while no significant difference was found in erythrocyte indices including mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) between the treatments. There is also no significant difference in former variables of the fish fed manganese sulfate and control diet. Findings of the present study showed that the use of nano manganese oxide increase some blood parameters of rainbow trout fingerlings including number of erythrocytes, hemoglobin, and hematocrit.

کلیدواژه‌ها [English]

  • Nanoparticles
  • Manganese
  • Rainbow trout
  • Growth performance
  • Blood cells
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